Journal: Pulmonary Circulation

Article Title: Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia

doi: 10.1002/pul2.12167

Figure Lengend Snippet: Endothelial‐specific knockout of β‐arrestins results in a retinal vascular development defect. (a) IB4 stained p7 retinal flat‐mounts of Arrb1/Arrb2 ECKO and wild‐type (control) mice. (b) Quantification of vascular radial expansion in Arrb1/Arrb2 ECKO and wild‐type (Ctl) mice. N = 7 wild‐type and 4 Arrb1/2 ECKO. *p < 0.05. (c) The number of branchpoints in Arrb1/Arrb2 ECKO and wild‐type (Ctl) mice. N = 7 wild‐type and 3 Arrb1/2 ECKO. *p < 0.05. (d) eNOS (green) and IB4 (red) stained p7 retinal flat‐mounts of Arrb1/2 ECKO and wild‐type mice. A indicates arteries and V indicates veins.

Article Snippet: The retinas were washed in PBS for 30 min at least eight times at room temperature and incubated with anti‐IB4 (I21412; Invitrogen; 1:200) and Alexa Fluor 488 goat anti‐rabbit IgG (A11008; Invitrogen; 1:500) as a secondary antibody for eNOS with 1% Triton X‐100 in PBS overnight at 4°C in the dark.

Techniques: Knock-Out, Staining

Journal: Scientific Reports

Article Title: Central expression of synaptophysin and synaptoporin in nociceptive afferent subtypes in the dorsal horn

doi: 10.1038/s41598-019-40967-y

Figure Lengend Snippet: Co-localization of synaptophysin and synaptoporin with IB4-binding C fibers in the thoracic dorsal horn. IB4 binding C fibers distributed in lamina II of T7 and T13 dorsal horns and expressed synaptophysin and/or synaptoporin as shown in a representative montage of confocal images from T7. ( A ) Areas of IB4 binding projections within the investigated region of interest were analyzed at both thoracic levels.( B ) Colocalization of IB4 binding axon terminals with synaptophysin or with synaptoporin was assessed by double labeling quantification ( C ) to find significantly different expression levels of synaptoporin and synaptophysin in this C fiber subpopulation (n = 4, p < 0.05, Kruskal-Wallis test with multiple comparison test, Cohen’s d > 1.3).

Article Snippet: The primary antibodies included goat anti-IB4 (1:400, Vector, Burlingame, CA), goat anti-CTB (1:2000, List Biological Laboratories, Campbell, CA), mouse anti-synaptophysin (1:1000, Synaptic Systems), rabbit anti-synaptoporin (1:300, Synaptic Systems), and Guinea pig anti-CGRP (1:2000, Peninsula Laboratories International, San Carlos, CA).

Techniques: Binding Assay, Labeling, Expressing

Journal: Scientific Reports

Article Title: Central expression of synaptophysin and synaptoporin in nociceptive afferent subtypes in the dorsal horn

doi: 10.1038/s41598-019-40967-y

Figure Lengend Snippet: Expression of synaptophysin and synaptoporin in dorsal cutaneous C fibers in the T7 and T13 spinal cord. Cutaneous C fibers were labeled with IB4 injections into DCNs. ( A ) DCN-specific C fibers projected laterally at the inner layer of laminae II overlaid by the synaptoporin distribution area as shown in representative montages of confocal images at T7. ( B ) Overlapping areas of DCN-specific IB4+ C fibers with synaptoporin and synaptophysin were quantified at every 200 μm across the sections centered at the dorsal horn entry zone (DREZ) at T7 and T13. ( C ) Bar graphs show averaged percent overlapping areas across those serial sections from 3 rats ( D ).

Article Snippet: The primary antibodies included goat anti-IB4 (1:400, Vector, Burlingame, CA), goat anti-CTB (1:2000, List Biological Laboratories, Campbell, CA), mouse anti-synaptophysin (1:1000, Synaptic Systems), rabbit anti-synaptoporin (1:300, Synaptic Systems), and Guinea pig anti-CGRP (1:2000, Peninsula Laboratories International, San Carlos, CA).

Techniques: Expressing, Labeling